Evaluation of men with symptoms or signs of possible hypogonadism, such as loss of libido, erectile dysfunction, gynecomastia, osteoporosis, or infertility Evaluation of boys with delayed or precocious puberty Monitoring testosterone replacement therapy Monitoring antiandrogen therapy (eg, used in prostate cancer, precocious puberty, treatment of idiopathic hirsutism, male-to-female transgender disorders, etc.) Evaluation of women with hirsutism, virilization, and oligoamenorrhea Evaluation of women with symptoms or signs of possible testosterone deficiency Evaluation of infants with ambiguous genitalia or virilization Diagnosis of androgen-secreting tumorsThe test is done to assess the level of Testosterone in blood. Circulating Testosterone consists of testosterone bound to SHBG and the bioavailable (Free & Bound to Albumin). The bioavailabile is biologically active. In men approximately 40% of Total testosterone is albumin bound while in women approximately 20% is albumin bound.