N-MYC by FISH

N-MYC (Neuroblastoma Myc) is a gene that plays a critical role in the regulation of cell growth and proliferation. Abnormalities in the N-MYC gene, such as gene amplification, are associated with the development of neuroblastoma, a childhood cancer that arises from immature nerve cells. Fluorescence In Situ Hybridization (FISH) is a molecular technique used to detect and assess N-MYC gene amplification in cancer cells, particularly in neuroblastoma. Here’s how N-MYC testing by FISH works:

• Sample Collection: A tissue sample, typically obtained through a biopsy or surgical procedure, is collected from the patient. This tissue sample contains neuroblastoma cells.
• Tissue Preparation: The collected tissue sample is processed and prepared for FISH analysis. This may involve fixing the tissue and preparing thin sections on glass slides.
• FISH Probe Preparation: Specific DNA probes are designed to target the N-MYC gene region. These probes are labeled with fluorescent molecules that emit distinct colors when exposed to specific wavelengths of light.
• Hybridization: The tissue sample is incubated with the DNA probes. If there is gene amplification in the N-MYC gene, meaning there are extra copies of it, the DNA probes will bind to the corresponding target sequences.
• Fluorescent Labeling: When the DNA probes bind to the target N-MYC gene sequences, they emit fluorescent signals. These signals can be visualized and analyzed using a fluorescent microscope.
• Imaging and Analysis: The tissue sample is examined under the fluorescent microscope. The number and pattern of fluorescent signals are assessed. The presence of multiple copies of the N-MYC gene, indicative of gene amplification, is identified based on the altered patterns of fluorescence signals.

N-MYC FISH testing is particularly important in the diagnosis and risk stratification of neuroblastoma, a cancer that affects children, often in early childhood. Gene amplification of N-MYC is associated with a more aggressive form of the disease and poorer prognosis. Detecting N-MYC gene amplification helps oncologists determine the appropriate treatment plan and predict the likelihood of the cancer responding to therapy.

The results of N-MYC FISH testing provide valuable information about the genetic characteristics of neuroblastoma cells and can guide treatment decisions. For patients with N-MYC-amplified neuroblastoma, more intensive therapies may be considered to improve treatment outcomes.

As with other FISH tests, the interpretation of N-MYC FISH results should be performed by experienced pathologists or oncologists who are familiar with the clinical context and guidelines for neuroblastoma. The results are often used in combination with other diagnostic and prognostic factors to tailor treatment plans for individual patients.